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SPAdes / Trinity running out of space still

#41Opendevjanee 创建于 2025-04-08
bug
D
devjaneecommented
### Description of the bug I'm running a bash script (attached below) and SPAdes and Trinity keep running out of space (html of latest run attached below). I changed the configs to what I believe to be enough CPUs and memory for SPAdes (according to SPAdes documentation), but it keeps failing I'm checking the latest run slurm output, and still getting the same error (attached) [slurm_output.zip](https://github.com/user-attachments/files/19648859/slurm_output.zip). Are there higher versions of than 1.2? (I'm looking at the system information below). ### Command used and terminal output ```console #!/bin/bash #SBATCH --job-name=spades_denovo #SBATCH --partition=common # Use 'scavenger' if needed #SBATCH --cpus-per-task=16 #SBATCH --mem=250G #SBATCH --time=7-00:00:00 #SBATCH --output=spades_%j.out #SBATCH --error=spades_%j.err conda activate env_nf nextflow run nf-core/denovotranscript \ --input Wisse_10855_150PE_samples.csv \ --outdir nextflow_output \ --max_memory 250.GB \ --max_cpus 16 \ --skip_fastp true \ --skip_fastq true \ --remove_ribo_rna true \ -resume ``` ### Relevant files [pipeline_info.zip](https://github.com/user-attachments/files/19648578/pipeline_info.zip) [nextflow.config.zip](https://github.com/user-attachments/files/19648601/nextflow.config.zip) [nf_denovo_transcript.sh.zip](https://github.com/user-attachments/files/19648602/nf_denovo_transcript.sh.zip) ### System information **Nextflow**: version 24.10.5, build 5935 (04-03-2025 17:55 UTC) **Hardware**: HPC **Executor**: slurm **Container**: conda **OS**: NAME="AlmaLinux" VERSION="9.5 (Teal Serval)" ID="almalinux" ID_LIKE="rhel centos fedora" VERSION_ID="9.5" PLATFORM_ID="platform:el9" PRETTY_NAME="AlmaLinux 9.5 (Teal Serval)" ANSI_COLOR="0;34" LOGO="fedora-logo-icon" CPE_NAME="cpe:/o:almalinux:almalinux:9::baseos" HOME_URL="https://almalinux.org/" DOCUMENTATION_URL="https://wiki.almalinux.org/" BUG_REPORT_URL="https://bugs.almalinux.org/" **Version of nf-core/denovotranscript:** ALMALINUX_MANTISBT_PROJECT="AlmaLinux-9" ALMALINUX_MANTISBT_PROJECT_VERSION="9.5" REDHAT_SUPPORT_PRODUCT="AlmaLinux" REDHAT_SUPPORT_PRODUCT_VERSION="9.5" SUPPORT_END=2032-06-01 project name: nf-core/denovotranscript repository : https://github.com/nf-core/denovotranscript local path : /hpc/home/ds394/.nextflow/assets/nf-core/denovotranscript main script : main.nf description : A pipeline for de novo transcriptome assembly of short reads from bulk RNA-seq author : Avani Bhojwani and Timothy Little revisions : * master (default) TEMPLATE dev nf-core-template-merge-3.0.0 nf-core-template-merge-3.0.2 1.0.0 [t] 1.1.0 [t] 1.2.0 [t]
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