ERROR in train module :

Hi, I'm pretty new to fungi and I discovered funannotate with a recent pangenome study on Aspegullus fumigatus (https://pubmed.ncbi.nlm.nih.gov/36395320/). I'm starting to work with _A. fumigatus_ and I have to annotated some genomes. so, i installed funannotate2 yesterday (CET time) -> funannotate2 v25.11.1 I'm on a linux cluster and i installed the conda env following the installation instructions in https://funannotate2.readthedocs.io/en/latest/installation.html `mambacreate -n funannotate2 gfftk gapmm2 minimap2 miniprot snap "augustus==3.5.0" glimmerhmm diamond trnascan-se table2asn gb-io buscolite conda activate funannotate2 python -m pip install git+https://github.com/nextgenusfs/funannotate2.git` I did not install GeneMark and i proceeded to test the different modules: 1- I defined a specific FUNANNOTATE-DB folder 2- I installed the databases (had to install mibig, go and dbCAN manually) 3 - test funannotate2 clean -> OK 4 - dowloaded BUSCO aspergillus_odb12.2025-07-01.tar.gz to FUNANNOTATE-DB/BUSCO 5 - funannotate2 train and here I have an error. this is the command I used `funannotate2 train -f /home/martinssimoes/bac_a_sable/analyses/funnnotate2/test_2025-02-13/Assemblies_cleaned/10_S10_cleaned_renamed.fasta -s "Aspergillus fumigatus" -o /home/martinssimoes/bac_a_sable/analyses/funnnotate2/test_2025-02-13 -t 24 --busco-lineage /path_folder_to_FUNANNOTATE2_DB/aspergillus_odb12` Here is the error : [Feb 13 05:50 PM] Python v3.12.12; funannotate2 v25.11.1; gfftk v25.6.10; buscolite v26.1.26 [Feb 13 05:50 PM] Loading genome assembly and running QC checks [Feb 13 05:50 PM] Genome stats: { "n_contigs": 777, "size": 28346551, "n50": 145342, "n90": 31443, "l50": 58, "l90": 212, "avg_length": 36482 } [Feb 13 05:50 PM] Filtered genome for training: kept 309 contigs (27,486,431 bp), filtered 468 contigs (860,120 bp) shorter than 10,000 bp ERROR in taxonomy lookup: ('Connection aborted.', ConnectionResetError(104, 'Connection reset by peer')) [Feb 13 05:50 PM] Getting taxonomy information false Traceback (most recent call last): File "/home/martinssimoes/miniconda3/envs/funannotate2/bin/funannotate2", line 6, in <module> sys.exit(main()) ^^^^^^ File "/home/martinssimoes/miniconda3/envs/funannotate2/lib/python3.12/site-packages/funannotate2/__main__.py", line 28, in main train(args) File "/home/martinssimoes/miniconda3/envs/funannotate2/lib/python3.12/site-packages/funannotate2/train.py", line 129, in train aug_species = choose_best_augustus_species(taxonomy) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "/home/martinssimoes/miniconda3/envs/funannotate2/lib/python3.12/site-packages/funannotate2/utilities.py", line 391, in choose_best_augustus_species return best_taxonomy(query_tax, augustus_species) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "/home/martinssimoes/miniconda3/envs/funannotate2/lib/python3.12/site-packages/funannotate2/utilities.py", line 480, in best_taxonomy score = similarity_score(query, attributes) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "/home/martinssimoes/miniconda3/envs/funannotate2/lib/python3.12/site-packages/funannotate2/utilities.py", line 431, in similarity_score return sum( ^^^^ File "/home/martinssimoes/miniconda3/envs/funannotate2/lib/python3.12/site-packages/funannotate2/utilities.py", line 434, in <genexpr> if level in query ^^^^^^^^^^^^^^ TypeError: argument of type 'bool' is not iterable It seems that it tries to connect to an external DB to check the taxonomy. But i thought that the augustus species was already downloaded ( miniconda3/envs/funannotate2/config/species). I verified `augustus --species=help ` and _Aspergillus fumigatus_ (as many other groups) appear listed. I am sure I'm doing something wrong and it's in front of my eyes... but i can't see it. do I need to set an AUGUSTUS path variable ? I didn't see it in the manual ... Thanks in advance for your help !! Best regards, Patricia