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Run Bigstream2 in Pipeline

#48Openanaste123 创建于 2024-01-21
bug
A
anaste123commented
## Bug report Hi, I was trying to do registration of our large image data using Bigsteam2 in EASI-FISH Pipeline, I changed most parameters exposed, including: --bigstream_global_steps --bigstream_local_steps --global_iterations --local_iterations --local_ransac_cc_radius --local_ransac_nspots --local_ransac_match_threshold --local_ransac_diagonal_constraint --aff_scale --def_scale --global_ransac_fix_spot_winsorize_limits --global_ransac_mov_spot_winsorize_limits --local_ransac_fix_spot_winsorize_limits --local_ransac_mov_spot_winsorize_limits I also tried the parameters you recommended before: ![屏幕截图 2024-01-21 152127](https://github.com/JaneliaSciComp/multifish/assets/87220455/1cbe7e69-4eab-4f1e-a484-2fb8f11da4df) ![fig2](https://github.com/JaneliaSciComp/multifish/assets/87220455/f4c2daf7-60f4-4474-b9af-508536641f3c) The same image can get good registration results using Bigstream2 Python script, but can not get good results using Bigstream2 in EASI-FISH Pipeline. From my experience, I think shrink_factors, smooth_sigmas from affine_kwargs/deform_kwargs have great influence on the registration results. Could you please expose those parameters in Registration step? Is there any other parameters exposed that I can use to improve the registration results? Thanks in advance! ## Description of the problem ## Log file(s) ### Environment * EASI-FISH Pipeline version: [dev] * Nextflow version: [23.04.1] * Container runtime: [Singularity] * Platform: [Slurm] * Operating system: [CentOS 7.6] ### Additional context
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