Perturbation Cell Atlas of Human Induced Pluripotent Stem Cells - Perturb Seq

ark:59853/rocrate-a-perturbation-cell-atlas-of-human-induced-pluripotent-stem-cells

This dataset represents an expressed genome-scale CRISPRi Perturbation Cell Atlas in KOLF2.1J human induced pluripotent stem cells (hiPSCs) mapping transcriptional and fitness phenotypes associated with 11,739 targeted genes, as part of the Cell Maps for Artificial Intelligence (CM4AI) Functional Genomics Grand Challenge, a component of the U.S. National Institute of Health's (NIH) Bridge2AI program. We validated these findings via phenotypic, protein-interaction, and metabolic tracing assays.

Doctor Y; Dailamy A; Forget A; Lee YH; Chinn B; Khaliq H; Polacco B; Muralidharan, M; Pan E; Zhang Y; Sigaeva A; Hansen JN; Gao J; Parker JA; Obernier K; Clark T; Chen JY; Metallo C; Lundberg E; Ideker T; Krogan N; Mali P

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Trey Ideker

pmali@ucsd.edu

https://creativecommons.org/licenses/by-nc-sa/4.0/deed.en

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AI, Artificial intelligence, Cell maps, CM4AI, CRISPR perturbation, IPSC, KOLF2.1J, Machine learning, Perturb-seq, scRNAseq, single-cell RNA sequencing

Clark T; Parker J; Al Manir S; Axelsson U; Ballllosero Navarro F; Chinn B; Churas CP; Dailamy A; Doctor Y; Fall J; Forget A; Gao J; Hansen JN; Hu M; Johannesson A; Khaliq H; Lee YH; Lenkiewicz J; Levinson MA; Marquez C; Metallo C; Muralidharan M; Nourreddine S; Niestroy J; Obernier K; Pan E; Polacco B; Pratt D; Qian G; Schaffer L; Sigaeva A; Thaker S; Zhang Y; Bélisle-Pipon JC; Brandt C; Chen JY; Ding Y; Fodeh S; Krogan N; Lundberg E; Mali P; Payne-Foster P; Ratcliffe S; Ravitsky V; Sali A; Schulz W; Ideker T, 2025, "Cell Maps for Artificial Intelligence - March 2025 Data Release (Beta)", https://doi.org/10.18130/V3/B35XWX , https://dataverse.lib.virginia.edu/, V1

• Nourreddine S, Doctor Y, Dailamy A, et al. (2024) "A Perturbation Cell Atlas of Human Induced Pluripotent Stem Cells" bioRxiv 2024.11.03.621734; doi: https://doi.org/10.1101/2024.11.03.621734