DIFFERENTIAL EXPRESSION · NSCLC

Differential Gene Expression Analysis

Fluoxetine-treated A549 and H1299 cells profiled against vehicle controls; 12,847 genes assayed, 629 differentially expressed at FDR < 0.05.

GSE200209 · A549 + H1299 · 24 h treatment · n=6 per arm

OVERVIEW

Expression Analysis Summary

Summary of differential expression analysis results from fluoxetine-treated NSCLC cell lines.

12,847
Total Genes
after filtering
342
Upregulated
padj < 0.05, log2FC > 1
287
Downregulated
padj < 0.05, log2FC < -1
4.8%
DEG Rate
629 / 12,847
Key Finding
Fluoxetine treatment induces significant ER stress response, with DDIT3 and ATF4 among the top upregulated genes (log2FC > 2.5). The unfolded protein response (UPR) pathway shows coordinated activation across multiple effectors, suggesting a potential mechanism for NSCLC cell growth inhibition.
Evidence High Confidence 3 Studies
VISUALIZATION

Volcano Plot

Log2 fold change vs. statistical significance for all genes.

Volcano Plot Red = upregulated, Blue = downregulated, Gray = not significant
CORTEX
DATA

Top Differentially Expressed Genes

Ranked by adjusted p-value. Click column headers to sort.

Gene log2FC padj baseMean Direction
DDIT3 3.42 1.2e-15 892 Up
ATF4 2.87 4.5e-12 1204 Up
HSPA5 2.31 2.1e-10 3421 Up
CCND1 -2.15 8.7e-09 2156 Down
MYC -1.94 3.2e-07 1876 Down
Pathway Scores
UPR / ER Stress ████████░░ 0.87
Apoptosis ██████░░░░ 0.64
Cell Cycle ████░░░░░░ 0.41