# Copyright 2009-2017 by Peter Cock.  All rights reserved.
# This code is part of the Biopython distribution and governed by its
# license.  Please see the LICENSE file that should have been included
# as part of this package.
"""SeqFeature related tests for SeqRecord objects from Bio.SeqIO.

Initially this takes matched tests of GenBank and FASTA files from the NCBI
and confirms they are consistent using our different parsers.
"""

import unittest

try:
    import numpy as np
except ImportError:
    np = None  # type: ignore

from Bio import SeqIO
from Bio.Seq import MutableSeq
from Bio.Seq import Seq
from Bio.SeqFeature import AfterPosition
from Bio.SeqFeature import BeforePosition
from Bio.SeqFeature import ExactPosition
from Bio.SeqFeature import OneOfPosition
from Bio.SeqFeature import SeqFeature
from Bio.SeqFeature import SimpleLocation
from Bio.SeqFeature import UnknownPosition
from Bio.SeqFeature import WithinPosition
from Bio.SeqRecord import SeqRecord


class SeqRecordCreation(unittest.TestCase):
    """Test basic creation of SeqRecords."""

    def test_annotations(self):
        """Pass in annotations to SeqRecords."""
        rec = SeqRecord(Seq("ACGT"), id="Test", name="Test", description="Test")
        self.assertEqual(rec.annotations, {})
        rec = SeqRecord(
            Seq("ACGT"),
            id="Test",
            name="Test",
            description="Test",
            annotations={"test": ["a test"]},
        )
        self.assertEqual(rec.annotations["test"], ["a test"])

    def test_letter_annotations(self):
        """Pass in letter annotations to SeqRecords."""
        rec = SeqRecord(Seq("ACGT"), id="Test", name="Test", description="Test")
        self.assertEqual(rec.annotations, {})
        rec = SeqRecord(
            Seq("ACGT"),
            id="Test",
            name="Test",
            description="Test",
            letter_annotations={"test": [1, 2, 3, 4]},
        )
        self.assertEqual(rec.letter_annotations["test"], [1, 2, 3, 4])
        # Now try modifying it to a bad value...
        try:
            rec.letter_annotations["bad"] = "abc"
            self.fail("Adding a bad letter_annotation should fail!")
        except (TypeError, ValueError) as e:
            pass
        # Now try setting it afterwards to a bad value...
        rec = SeqRecord(Seq("ACGT"), id="Test", name="Test", description="Test")
        try:
            rec.letter_annotations = {"test": [1, 2, 3]}
            self.fail("Changing to bad letter_annotations should fail!")
        except (TypeError, ValueError) as e:
            pass
        # Now try setting it at creation time to a bad value...
        try:
            rec = SeqRecord(
                Seq("ACGT"),
                id="Test",
                name="Test",
                description="Test",
                letter_annotations={"test": [1, 2, 3]},
            )
            self.fail("Wrong length letter_annotations should fail!")
        except (TypeError, ValueError) as e:
            pass

    def test_replacing_seq(self):
        """Replacing .seq if .letter_annotation present."""
        rec = SeqRecord(
            Seq("ACGT"),
            id="Test",
            name="Test",
            description="Test",
            letter_annotations={"example": [1, 2, 3, 4]},
        )
        try:
            rec.seq = Seq("ACGTACGT")
            self.fail(
                "Changing .seq length with letter_annotations present should fail!"
            )
        except ValueError as e:
            self.assertEqual(str(e), "You must empty the letter annotations first!")
        # Check we can replace IF the length is the same
        self.assertEqual(rec.seq, "ACGT")
        self.assertEqual(rec.letter_annotations, {"example": [1, 2, 3, 4]})
        rec.seq = Seq("NNNN")
        self.assertEqual(rec.seq, "NNNN")
        self.assertEqual(rec.letter_annotations, {"example": [1, 2, 3, 4]})

    def test_valid_id(self):
        with self.assertRaises(TypeError):
            SeqRecord(Seq("ACGT"), id={})

    def test_valid_name(self):
        with self.assertRaises(TypeError):
            SeqRecord(Seq("ACGT"), name={})

    def test_valid_seq(self):
        with self.assertRaises(TypeError):
            SeqRecord("ACGT")

    def test_valid_description(self):
        with self.assertRaises(TypeError):
            SeqRecord(Seq("ACGT"), description={})

    def test_valid_dbxrefs(self):
        with self.assertRaises(TypeError):
            SeqRecord(Seq("ACGT"), dbxrefs={})

    def test_valid_annotations(self):
        with self.assertRaises(TypeError):
            SeqRecord(Seq("ACGT"), annotations=[])

    def test_valid_features(self):
        with self.assertRaises(TypeError):
            SeqRecord(Seq("ACGT"), features={})

    def test_default_properties(self):
        seqobj = Seq("A")
        default__dict__ = {
            "_seq": seqobj,
            "id": "<unknown id>",
            "name": "<unknown name>",
            "description": "<unknown description>",
            "dbxrefs": [],
            "annotations": {},
            "_per_letter_annotations": None,
            "features": [],
        }
        bsr = SeqRecord(seqobj)
        self.assertEqual(bsr.__dict__, default__dict__)


class SeqRecordMethods(unittest.TestCase):
    """Test SeqRecord methods."""

    def setUp(self):
        f0 = SeqFeature(
            SimpleLocation(0, 26),
            type="source",
            qualifiers={"mol_type": ["fake protein"]},
        )
        f1 = SeqFeature(SimpleLocation(0, ExactPosition(10)))
        f2 = SeqFeature(
            SimpleLocation(WithinPosition(12, left=12, right=15), BeforePosition(22))
        )
        f3 = SeqFeature(
            SimpleLocation(
                AfterPosition(16),
                OneOfPosition(26, [ExactPosition(25), AfterPosition(26)]),
            )
        )
        self.record = SeqRecord(
            Seq("ABCDEFGHIJKLMNOPQRSTUVWZYX"),
            id="TestID",
            name="TestName",
            description="TestDescr",
            dbxrefs=["TestXRef"],
            annotations={"k": "v"},
            letter_annotations={"fake": "X" * 26},
            features=[f0, f1, f2, f3],
        )

    def test_iter(self):
        for amino in self.record:
            self.assertEqual("A", amino)
            break

    def test_contains(self):
        self.assertIn(Seq("ABC"), self.record)

    def test_bytes(self):
        self.assertEqual(b"ABCDEFGHIJKLMNOPQRSTUVWZYX", bytes(self.record))

    def test_str(self):
        expected = """
ID: TestID
Name: TestName
Description: TestDescr
Database cross-references: TestXRef
Number of features: 4
/k=v
Per letter annotation for: fake
Seq('ABCDEFGHIJKLMNOPQRSTUVWZYX')"""
        self.assertEqual(expected.lstrip(), str(self.record))

    def test_repr(self):
        expected = (
            "SeqRecord(seq=Seq('ABCDEFGHIJKLMNOPQRSTUVWZYX'), "
            "id='TestID', name='TestName', description='TestDescr', dbxrefs=['TestXRef'])"
        )
        self.assertEqual(expected, repr(self.record))

    def test_format(self):
        expected = ">TestID TestDescr\nABCDEFGHIJKLMNOPQRSTUVWZYX\n"
        self.assertEqual(expected, self.record.format("fasta"))

    def test_format_str(self):
        expected = ">TestID TestDescr\nABCDEFGHIJKLMNOPQRSTUVWZYX\n"
        self.assertEqual(expected, f"{self.record:fasta}")

    def test_format_str_binary(self):
        with self.assertRaisesRegex(
            ValueError, "Binary format sff cannot be used with SeqRecord format method"
        ):
            f"{self.record:sff}"

    def test_format_spaces(self):
        rec = SeqRecord(
            Seq("ABCDEFGHIJKLMNOPQRSTUVWZYX"),
            id="TestID",
            name="TestName",
            description="TestDescr",
        )
        rec.description = "TestDescr     with5spaces"
        expected = ">TestID TestDescr     with5spaces\nABCDEFGHIJKLMNOPQRSTUVWZYX\n"
        self.assertEqual(expected, rec.format("fasta"))

    def test_count(self):
        self.assertEqual(self.record.count("HIJK"), 1)
        self.assertRaises(TypeError, SeqRecord(Seq("AC777GT")).count, 7)
        self.assertRaises(TypeError, SeqRecord(Seq("AC777GT")).count, None)

    def test_upper(self):
        self.assertEqual("ABCDEFGHIJKLMNOPQRSTUVWZYX", self.record.lower().upper().seq)
        seqobj = Seq("A")
        default__dict__ = {
            "_seq": seqobj,
            "id": "<unknown id>",
            "name": "<unknown name>",
            "description": "<unknown description>",
            "dbxrefs": [],
            "annotations": {},
            "_per_letter_annotations": None,
            "features": [],
        }
        bsr = SeqRecord(seqobj)
        bsru = bsr.upper()
        self.assertEqual(bsru.__dict__, default__dict__)

    def test_lower(self):
        self.assertEqual("abcdefghijklmnopqrstuvwzyx", self.record.lower().seq)

    def test_isupper(self):
        self.assertTrue(self.record.isupper())
        self.assertFalse(self.record.lower().isupper())

    def test_islower(self):
        self.assertFalse(self.record.islower())
        self.assertTrue(self.record.lower().islower())

    def test_slicing(self):
        self.assertEqual("B", self.record[1])
        self.assertEqual("BC", self.record[1:3].seq)
        with self.assertRaises(ValueError):
            c = self.record["a"].seq
        if np is not None:
            start, stop = np.array([1, 3])  # numpy integers
            self.assertEqual("B", self.record[start])
            self.assertEqual("BC", self.record[start:stop].seq)

    def test_slice_variants(self):
        """Simple slices using different start/end values."""
        for start in list(range(-30, 30)) + [None]:
            for end in list(range(-30, 30)) + [None]:
                if start is None and end is None:
                    continue
                rec = self.record[start:end]
                seq = self.record.seq[start:end]
                seq_str = str(self.record.seq)[start:end]
                self.assertEqual(seq_str, str(seq))
                self.assertEqual(seq_str, str(rec.seq))
                self.assertEqual("X" * len(seq_str), rec.letter_annotations["fake"])

    def test_slice_simple(self):
        """Simple slice."""
        rec = self.record
        self.assertEqual(len(rec), 26)
        left = rec[:10]
        self.assertEqual(left.seq, rec.seq[:10])
        right = rec[-10:]
        self.assertEqual(right.seq, rec.seq[-10:])
        mid = rec[12:22]
        self.assertEqual(mid.seq, rec.seq[12:22])
        for sub in [left, right, mid]:
            self.assertEqual(len(sub), 10)
            self.assertEqual(sub.id, "TestID")
            self.assertEqual(sub.name, "TestName")
            self.assertEqual(sub.description, "TestDescr")
            self.assertEqual(sub.letter_annotations, {"fake": "X" * 10})
            self.assertEqual(sub.dbxrefs, [])  # May change this...
            self.assertEqual(sub.annotations, {})  # May change this...
            self.assertEqual(len(sub.features), 1)
            # By construction, each feature matches the full sliced region:
            self.assertEqual(sub.features[0].extract(sub.seq), sub.seq)
            self.assertEqual(sub.features[0].extract(sub.seq), sub.seq)

    def test_slice_zero(self):
        """Zero slice."""
        rec = self.record
        self.assertEqual(len(rec), 26)
        self.assertEqual(len(rec[2:-2]), 22)
        self.assertEqual(len(rec[5:2]), 0)
        self.assertEqual(len(rec[5:2][2:-2]), 0)

    def test_add_simple(self):
        """Simple addition."""
        rec = self.record + self.record
        self.assertEqual(len(rec), 52)
        self.assertEqual(rec.id, "TestID")
        self.assertEqual(rec.name, "TestName")
        self.assertEqual(rec.description, "TestDescr")
        self.assertEqual(rec.dbxrefs, ["TestXRef"])
        self.assertEqual(rec.annotations, {"k": "v"})
        self.assertEqual(rec.letter_annotations, {"fake": "X" * 52})
        self.assertEqual(len(rec.features), 2 * len(self.record.features))

    def test_add_seq(self):
        """Simple addition of Seq or string."""
        for other in [Seq("BIO"), "BIO"]:
            rec = self.record + other  # will use SeqRecord's __add__ method
            self.assertEqual(len(rec), 26 + 3)
            self.assertEqual(rec.seq, str(self.record.seq) + "BIO")
            self.assertEqual(rec.id, "TestID")
            self.assertEqual(rec.name, "TestName")
            self.assertEqual(rec.description, "TestDescr")
            self.assertEqual(rec.dbxrefs, ["TestXRef"])
            self.assertEqual(rec.annotations, {"k": "v"})
            self.assertEqual(rec.letter_annotations, {})
            self.assertEqual(len(rec.features), len(self.record.features))
            self.assertEqual(rec.features[0].type, "source")
            self.assertEqual(rec.features[0].location.start, 0)
            self.assertEqual(rec.features[0].location.end, 26)  # not +3

    def test_add_seqrecord(self):
        """Simple left addition of SeqRecord from genbank file."""
        other = SeqIO.read("GenBank/dbsource_wrap.gb", "gb")
        other.dbxrefs = ["dummy"]
        rec = self.record + other
        self.assertEqual(len(rec), len(self.record) + len(other))
        self.assertEqual(rec.seq, self.record.seq + other.seq)
        self.assertEqual(rec.id, "<unknown id>")
        self.assertEqual(rec.name, "<unknown name>")
        self.assertEqual(rec.description, "<unknown description>")
        self.assertEqual(rec.dbxrefs, ["TestXRef", "dummy"])
        self.assertEqual(len(rec.annotations), 0)
        self.assertEqual(len(rec.letter_annotations), 0)
        self.assertEqual(
            len(rec.features), len(self.record.features) + len(other.features)
        )
        self.assertEqual(rec.features[0].type, "source")
        self.assertEqual(rec.features[0].location.start, 0)
        self.assertEqual(rec.features[0].location.end, len(self.record))  # not +3
        i = len(self.record.features)
        self.assertEqual(rec.features[i].type, "source")
        self.assertEqual(rec.features[i].location.start, len(self.record))
        self.assertEqual(rec.features[i].location.end, len(rec))

    def test_add_seq_left(self):
        """Simple left addition of Seq or string."""
        for other in [Seq("BIO"), "BIO"]:
            rec = other + self.record  # will use SeqRecord's __radd__ method
            self.assertEqual(len(rec), 26 + 3)
            self.assertEqual(rec.seq, "BIO" + self.record.seq)
            self.assertEqual(rec.id, "TestID")
            self.assertEqual(rec.name, "TestName")
            self.assertEqual(rec.description, "TestDescr")
            self.assertEqual(rec.dbxrefs, ["TestXRef"])
            self.assertEqual(rec.annotations, {"k": "v"})
            self.assertEqual(rec.letter_annotations, {})
            self.assertEqual(len(rec.features), len(self.record.features))
            self.assertEqual(rec.features[0].type, "source")
            self.assertEqual(rec.features[0].location.start, 3)
            self.assertEqual(rec.features[0].location.end, 26 + 3)

    def test_slice_add_simple(self):
        """Simple slice and add."""
        for cut in range(27):
            rec = self.record[:cut] + self.record[cut:]
            self.assertEqual(rec.seq, self.record.seq)
            self.assertEqual(len(rec), 26)
            self.assertEqual(rec.id, "TestID")
            self.assertEqual(rec.name, "TestName")
            self.assertEqual(rec.description, "TestDescr")
            self.assertEqual(rec.dbxrefs, [])  # May change this...
            self.assertEqual(rec.annotations, {})  # May change this...
            self.assertEqual(rec.letter_annotations, {"fake": "X" * 26})
            self.assertLessEqual(len(rec.features), len(self.record.features))

    def test_slice_add_shift(self):
        """Simple slice and add to shift."""
        for cut in range(27):
            rec = self.record[cut:] + self.record[:cut]
            self.assertEqual(rec.seq, self.record.seq[cut:] + self.record.seq[:cut])
            self.assertEqual(len(rec), 26)
            self.assertEqual(rec.id, "TestID")
            self.assertEqual(rec.name, "TestName")
            self.assertEqual(rec.description, "TestDescr")
            self.assertEqual(rec.dbxrefs, [])  # May change this...
            self.assertEqual(rec.annotations, {})  # May change this...
            self.assertEqual(rec.letter_annotations, {"fake": "X" * 26})
            self.assertLessEqual(len(rec.features), len(self.record.features))


class SeqRecordMethodsMore(unittest.TestCase):
    """Test SeqRecord methods cont."""

    # This class does not have a setUp defining self.record

    def test_reverse_complement_seq(self):
        s = SeqRecord(
            Seq("ACTG"),
            id="TestID",
            name="TestName",
            description="TestDescription",
            dbxrefs=["TestDbxrefs"],
            features=[SeqFeature(SimpleLocation(0, 3), type="Site")],
            annotations={"organism": "bombyx"},
            letter_annotations={"test": "abcd"},
        )
        rc = s.reverse_complement(
            id=True,
            name=True,
            description=True,
            dbxrefs=True,
            features=True,
            annotations=True,
            letter_annotations=True,
        )

        self.assertEqual("CAGT", rc.seq)
        self.assertEqual("TestID", rc.id)
        self.assertEqual("TestID", s.reverse_complement(id="TestID").id)

        self.assertEqual("TestName", rc.name)
        self.assertEqual("TestName", s.reverse_complement(name="TestName").name)

        self.assertEqual("TestDescription", rc.description)
        self.assertEqual(
            "TestDescription",
            s.reverse_complement(description="TestDescription").description,
        )

        self.assertEqual(["TestDbxrefs"], rc.dbxrefs)
        self.assertEqual(
            ["TestDbxrefs"], s.reverse_complement(dbxrefs=["TestDbxrefs"]).dbxrefs
        )

        self.assertEqual(
            "[SeqFeature(SimpleLocation(ExactPosition(1), ExactPosition(4)), type='Site')]",
            repr(rc.features),
        )
        rc2 = s.reverse_complement(
            features=[SeqFeature(SimpleLocation(1, 4), type="Site")]
        )
        self.assertEqual(
            "[SeqFeature(SimpleLocation(ExactPosition(1), ExactPosition(4)), type='Site')]",
            repr(rc2.features),
        )

        self.assertEqual({"organism": "bombyx"}, rc.annotations)
        self.assertEqual(
            {"organism": "bombyx"},
            s.reverse_complement(annotations={"organism": "bombyx"}).annotations,
        )

        self.assertEqual({"test": "dcba"}, rc.letter_annotations)
        self.assertEqual(
            {"test": "abcd"},
            s.reverse_complement(
                letter_annotations={"test": "abcd"}
            ).letter_annotations,
        )

    def test_reverse_complement_mutable_seq(self):
        s = SeqRecord(MutableSeq("ACTG"))
        self.assertEqual("CAGT", s.reverse_complement().seq)

    def test_reverse_complement_feature_order_with_unknown_positions(self):
        features = [
            SeqFeature(SimpleLocation(1, UnknownPosition()), type="unknown-a"),
            SeqFeature(SimpleLocation(2, 10), type="equal-a"),
            SeqFeature(SimpleLocation(3, UnknownPosition()), type="unknown-b"),
            SeqFeature(SimpleLocation(1, 5), type="known-late"),
            SeqFeature(SimpleLocation(10, 15), type="known-early"),
            SeqFeature(SimpleLocation(3, 10), type="equal-b"),
            SeqFeature(SimpleLocation(UnknownPosition(), 12), type="known-middle"),
        ]
        record = SeqRecord(Seq("A" * 20), features=features)

        reverse = record.reverse_complement()

        self.assertEqual(
            [
                "known-early",
                "known-middle",
                "equal-a",
                "equal-b",
                "known-late",
                "unknown-a",
                "unknown-b",
            ],
            [feature.type for feature in reverse.features],
        )

    def test_translate(self):
        s = SeqRecord(
            Seq("ATGGTGTAA"),
            id="TestID",
            name="TestName",
            description="TestDescription",
            dbxrefs=["TestDbxrefs"],
            features=[SeqFeature(SimpleLocation(0, 3), type="Site")],
            annotations={"organism": "bombyx"},
            letter_annotations={"test": "abcdefghi"},
        )

        t = s.translate()
        self.assertEqual(t.seq, "MV*")
        self.assertEqual(t.id, "<unknown id>")
        self.assertEqual(t.name, "<unknown name>")
        self.assertEqual(t.description, "<unknown description>")
        self.assertFalse(t.dbxrefs)
        self.assertFalse(t.features)
        self.assertEqual(t.annotations, {"molecule_type": "protein"})
        self.assertFalse(t.letter_annotations)

        t = s.translate(
            cds=True,
            id=True,
            name=True,
            description=True,
            dbxrefs=True,
            annotations=True,
        )
        self.assertEqual(t.seq, "MV")
        self.assertEqual(t.id, "TestID")
        self.assertEqual(t.name, "TestName")
        self.assertEqual(t.description, "TestDescription")
        self.assertEqual(t.dbxrefs, ["TestDbxrefs"])
        self.assertFalse(t.features)
        self.assertEqual(
            t.annotations, {"organism": "bombyx", "molecule_type": "protein"}
        )
        self.assertFalse(t.letter_annotations)

    def test_no_side_effects(self):
        a = SeqRecord(Seq("AAA"))
        self.assertIsNone(a._per_letter_annotations)
        a.reverse_complement()
        self.assertIsNone(a._per_letter_annotations)

        a = SeqRecord(Seq("AAA"))
        self.assertIsNone(a._per_letter_annotations)
        a.translate()
        self.assertIsNone(a._per_letter_annotations)

    def test_lt_exception(self):
        def lt():
            return SeqRecord(Seq("A")) < SeqRecord(Seq("A"))

        self.assertRaises(NotImplementedError, lt)

    def test_le_exception(self):
        def le():
            return SeqRecord(Seq("A")) <= SeqRecord(Seq("A"))  # type: ignore

        self.assertRaises(NotImplementedError, le)

    def test_eq_exception(self):
        def equality():
            return SeqRecord(Seq("A")) == SeqRecord(Seq("A"))  # type: ignore

        self.assertRaises(NotImplementedError, equality)

    def test_ne_exception(self):
        def notequality():
            return SeqRecord(Seq("A")) != SeqRecord(Seq("A"))  # type: ignore

        self.assertRaises(NotImplementedError, notequality)

    def test_gt_exception(self):
        def gt():
            return SeqRecord(Seq("A")) > SeqRecord(Seq("A"))  # type: ignore

        self.assertRaises(NotImplementedError, gt)

    def test_ge_exception(self):
        def ge():
            return SeqRecord(Seq("A")) >= SeqRecord(Seq("A"))  # type: ignore

        self.assertRaises(NotImplementedError, ge)

    def test_hash_exception(self):
        def hash1():
            hash(SeqRecord(Seq("A")))

        self.assertRaises(TypeError, hash1)

        def hash2():
            SeqRecord(Seq("A")).__hash__()

        self.assertRaises(TypeError, hash2)


class TestTranslation(unittest.TestCase):
    def setUp(self):
        self.s = SeqRecord(
            Seq("ATGGTGTAA"),
            id="TestID",
            name="TestName",
            description="TestDescription",
            dbxrefs=["TestDbxrefs"],
            features=[SeqFeature(SimpleLocation(0, 3), type="Site")],
            annotations={"organism": "bombyx"},
            letter_annotations={"test": "abcdefghi"},
        )

    def test_defaults(self):
        t = self.s.translate()
        self.assertEqual(t.seq, "MV*")
        self.assertEqual(t.id, "<unknown id>")
        self.assertEqual(t.name, "<unknown name>")
        self.assertEqual(t.description, "<unknown description>")
        self.assertFalse(t.dbxrefs)
        self.assertFalse(t.features)
        self.assertEqual(t.annotations, {"molecule_type": "protein"})
        self.assertFalse(t.letter_annotations)

    def test_preserve(self):
        t = self.s.translate(
            cds=True,
            id=True,
            name=True,
            description=True,
            dbxrefs=True,
            annotations=True,
        )
        self.assertEqual(t.seq, "MV")
        self.assertEqual(t.id, "TestID")
        self.assertEqual(t.name, "TestName")
        self.assertEqual(t.description, "TestDescription")
        self.assertEqual(t.dbxrefs, ["TestDbxrefs"])
        self.assertFalse(t.features)
        self.assertEqual(
            t.annotations, {"organism": "bombyx", "molecule_type": "protein"}
        )
        self.assertFalse(t.letter_annotations)

        # Should not preserve these
        self.assertRaises(TypeError, self.s.translate, features=True)
        self.assertRaises(TypeError, self.s.translate, letter_annotations=True)

    def test_new_annot(self):
        t = self.s.translate(
            1,
            to_stop=True,
            gap="-",
            id="Foo",
            name="Bar",
            description="Baz",
            dbxrefs=["Nope"],
            features=[SeqFeature(SimpleLocation(0, 3), type="Site")],
            annotations={"a": "team"},
            letter_annotations={"aa": ["Met", "Val"]},
        )
        self.assertEqual(t.seq, "MV")
        self.assertEqual(t.id, "Foo")
        self.assertEqual(t.name, "Bar")
        self.assertEqual(t.description, "Baz")
        self.assertEqual(t.dbxrefs, ["Nope"])
        self.assertEqual(len(t.features), 1)
        self.assertEqual(t.annotations, {"a": "team", "molecule_type": "protein"})
        self.assertEqual(t.letter_annotations, {"aa": ["Met", "Val"]})


class DerivedRecordIsolation(unittest.TestCase):
    """A record derived from another must not share its mutable annotations.

    Each method returns a new record; mutating that record's annotations, or a
    mutable value inside them, must leave the original untouched.
    """

    def setUp(self):
        self.parent = SeqRecord(
            Seq("ACGTACGT"),
            id="parent",
            annotations={"keywords": ["a", "b"], "molecule_type": "DNA"},
        )

    def _assert_isolated(self, derived):
        derived.annotations["keywords"].append("EVIL")
        self.assertEqual(
            self.parent.annotations["keywords"],
            ["a", "b"],
            "mutating the derived record's keywords changed the parent's",
        )

    def test_add_string_isolates_annotations(self):
        self._assert_isolated(self.parent + "TT")

    def test_radd_string_isolates_annotations(self):
        self._assert_isolated("TT" + self.parent)

    def test_add_two_records_isolates_shared_annotations(self):
        """Merging two records must not share the common annotation values.

        The merge keeps an annotation only when both records agree on it, and
        used to store the left record's value object directly, so the merged
        record shared it with both parents.
        """
        other = SeqRecord(
            Seq("TTTT"),
            id="other",
            annotations={"keywords": ["a", "b"], "molecule_type": "DNA"},
        )
        combined = self.parent + other
        self.assertEqual(combined.annotations["keywords"], ["a", "b"])
        combined.annotations["keywords"].append("EVIL")
        self.assertEqual(self.parent.annotations["keywords"], ["a", "b"])
        self.assertEqual(other.annotations["keywords"], ["a", "b"])

    def test_upper_isolates_annotations(self):
        self._assert_isolated(self.parent.upper())

    def test_lower_isolates_annotations(self):
        self._assert_isolated(self.parent.lower())

    def test_reverse_complement_isolates_annotations(self):
        self._assert_isolated(self.parent.reverse_complement(annotations=True))

    def test_translate_isolates_annotations(self):
        coding = SeqRecord(
            Seq("ATGGTGTAA"),
            id="coding",
            annotations={"keywords": ["a", "b"], "molecule_type": "DNA"},
        )
        derived = coding.translate(annotations=True)
        derived.annotations["keywords"].append("EVIL")
        self.assertEqual(coding.annotations["keywords"], ["a", "b"])

    def test_uncopyable_annotation_value_does_not_break_derivation(self):
        """A value that cannot be deep-copied must not stop a record deriving.

        ``SeqRecord`` only requires annotations to be a dict, so a value such
        as an open file handle is legal. Deep-copying it raises; the helper
        falls back to sharing it, exactly as the old shallow copy did, rather
        than turning a previously working ``upper()`` into an error.
        """
        with open(__file__) as handle:
            rec = SeqRecord(Seq("ACGT"), annotations={"source": handle})
            derived = rec.upper()
            self.assertIs(derived.annotations["source"], handle)

    def test_concatenate_letter_annotation_typeerror_names_the_key(self):
        """A failed per-letter concatenation reports the key and types."""
        left = SeqRecord(Seq("AC"), letter_annotations={"q": [1, 2]})
        right = SeqRecord(Seq("GT"), letter_annotations={"q": "xy"})
        with self.assertRaisesRegex(TypeError, "per-letter annotation 'q'"):
            left + right


if __name__ == "__main__":
    runner = unittest.TextTestRunner(verbosity=2)
    unittest.main(testRunner=runner)
